Native Human Collagen Type I

• Cat.No.: Collagen Type I-04H
• Product Overview: Human Collagen Type I is isolated from human neo-natal fibroblast cells.
  Gelation Time: <90 mins
  Fibril Formation: >0.5 abs. units
  pH: approx 2
  Telo vs. Atelo: Atelo-collagen

Specification

• Description: These cells are cultured in optimal conditions allowing these cells to naturally and efficiently secrete extracellular matrix which is processed and purified to produce human collagen. Manufactured under stringent quality standards with high lot-to-lot consistency. Made up of 97% Type I human collagen and 3% Type III collagen.
• Source: Neo-Natal Fibroblast Cells
• Species: Human
• Endotoxin: ≤1.00 EU/mL as measured by kinetic LAL
• Purity: >99.9%
• Applications: 3D Gels, Tissue engineering research, Wound healing constructs, Coatings, Solid free-form fabrications, Electrospinning, in-vitro research, Nerve Repair, Hemostats, Sealants, Coils, Sponges, Spheres/Beads, Ligaments, Strings, Tubes/Cylinders, Films
• Usage: Coating Procedure
  Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
  1. Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
  2. Dilute in water to ~50 to 100 μg/mL (~1:30). A 0.01 N HCl solution may also be used.
  3. Swirl contents gently until material is completely mixed.
  4. Add appropriate amount of diluted collagen material to the culture surface ensuring that the entire surface is coated.
  5. Incubate at room temperature or 37 centigrade, covered, for 1-2 hours.
  6. After incubation, aspirate any remaining material.
  7. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
  8. Coated surfaces are ready for use. They may also be stored at 2-8 centigrade damp or air dried if sterility is maintained.
  3-D Gel Preparation Procedure
  1. Slowly add 1 part of chilled 10× PBS or 10× culture media to 8 parts of chilled collagen solution with gentle swirling.
  2. Adjust pH of mixture to 7.2-7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
  3. Adjust final volume to a total of 10 parts with sterile water.
  4. To prevent gelation, maintain temperature of mixture at 2-10 centigrade.
  5. To form gel, warm to 37 centigrade. Allow approximately 120 minutes for gel formation.
• Storage: 4 centigrade
• Concentration: 2.9-3.2 mg/mL
• Storage Buffer: 0.01 N HCl (pH 2)

Gene Information

• Official Symbol: Collagen Type I
• Synonyms: Collagen Type I