Native Horse gram Dolichos biflorus Lectin (DBA), TRITC-Conjugated

• Cat.No.: DBA-488H
• Product Overview: Native Horse gram Dolichos biflorus Lectin (DBA) - TRITC (Rhodamine)
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: α-N-Acetylgalactosamine
  Inhibiting or Eluting Sugar: N-Acetylgalactosamine
  Divalent Ions: Ca++, Mn++, Mg++, Zn++
  Excitation: 545-555 nm
  Emission: 570-580 nm
  Mitogenic Activity: No
  Hazardous Shipping: Non-hazardous
  Fluorescence: Orange

Specification

• Species: Horse gram
• Source: Horse gram
• Description: Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4. 6 and pH 5. 5. DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances. Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding. Dolichos biflorus Lectin (DBA) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled DBA can be used for cellular imaging applications.
• Form: Liquid
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: At 2-8 centigrade.
• Concentration: 5 mg/mL

Gene Information

• Official Symbol: DBA
• Synonyms: Dolichos biflorus Lectin; DBA