Native Horse gram Dolichos biflorus Lectin (DBA), FITC-Conjugated

• Cat.No.: DBA-303H
• Product Overview: Native Horse gram Dolichos biflorus Lectin (DBA) - FITC (Fluorescein)
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: α-N-Acetylgalactosamine
  Inhibiting or Eluting Sugar: N-Acetylgalactosamine
  Divalent Ions: Ca++, Mn++, Mg++, Zn++
  Excitation: 495 nm
  Emission: 515 nm
  Mitogenic Activity: No
  Hazardous Shipping: Non-hazardous
  Fluorescence: Green

Specification

• Species: Horse gram
• Source: Horse gram
• Description: Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4. 6 and pH 5. 5. DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances. Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding. Dolichos biflorus Lectin (DBA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.
• Form: Liquid
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: At 2-8 centigrade.
• Concentration: 5 mg/mL

Gene Information

• Official Symbol: DBA
• Synonyms: Dolichos biflorus Lectin; DBA