NADP-Malate Dehydrogenase Assay Kit II

• Cat.No.: KITZ-048
• Product Overview: MDH(EC 1.1.1.37)is widely exist in animal, plant, microbe and cells culture. MDH in mitochondria is
  one of the key enzymes of TCA cycle, which catalyzes the formation of oxaloacetic acid from malic acid.
  In contrast, MDH in serum catalyzes the formation of malic acid from oxaloacetic acid. Oxaloacetic acid is
  an important intermediate that connects several important metabolic pathways. MDH plays an important
  role in many physiological activities of cells, including mitochondrial energy metabolism, malic
  acid-aspartic acid shuttle system, reactive oxygen species metabolism and disease resistance. According to
  the different coenzyme specificity, MDH is divided into NAD- dependent MDH and NADP- dependent
  MDH. NADP-MDH is mainly present in eukaryotic cells.
  NADP-MDH catalyzes NADPH to reduce oxaloacetic acid into malic acid, resulting in a decrease in
  absorption at 340 nm.

Specification

• Size: 100T/96S
• Storage: Store at -20 °C
• Shipping: Ice pack
• Kit Components: Extract solution: Liquid 100 mL×1, store at 4°C;
  Reagent I: Liquid 20 mL×1, store at 4°C;
  Reagent II: Powder×1, store at -20°C. Add 500 μL distilled water when the solution will be used. The rest
  of reagent store at -20°C;
  Reagent III: Powder×1, store at -20°C. Add 600 μL distilled water when the solution will be used. The rest
  of reagent store at -20°C
• Materials Required but Not Supplied: Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath, adjustablepipette, micro
  quartz cuvette/96-well flat-bottomUV plate and distilled water.