NAD-Malate Dehydrogenase Activity Assay Kit II

• Cat.No.: KITZ-046
• Product Overview: Malate Dehydrogenase (MDH, EC 1.1.1.37)widely exist in animal, plant, microbe and cells culture. MDH
  in mitochondria is one of the key enzymes of TCA cycle, which catalyzes the formation of oxaloacetic acid
  from malic acid. In contrast, MDH in serum catalyzes the formation of malic acid from oxaloacetic acid.
  Oxaloacetic acid is an important intermediate that connects several important metabolic pathways. MDH
  plays an important role in many physiological activities of cells, including mitochondrial energy
  metabolism, malic acid-aspartic acid shuttle system, reactive oxygen species metabolism and disease
  resistance. According to the different coenzyme specificity, MDH is divided into NAD- dependent MDH
  and NADP- dependent MDH. Bacteria usually only contain NAD-MDH. NAD-MDH is distributed in the
  cytoplasm and mitochondria in eukaryotic cells.
  NAD-MDH catalyzes NADH to reduce oxaloacetic acid into malic acid, resulting in the decrease of light
  absorption at 340nm.

Specification

• Size: 100T/96S
• Storage: Store at -20 °C
• Shipping: Ice pack
• Kit Components: Extract solution: Liquid 100 mL×1, store at 4°C.
  Reagent I: Liquid 20 mL×1, store at 4°C.
  Reagent II: Powder×1, store at -20°C. Working solution: add 360 μL of distilled water when the solution
  will be used. The rest of reagent store at -20°C.
  Reagent III: Powder×1, store at -20°C. Working solution: add 327 μL of distilled waterwhen the solution
  will be used. The rest of reagent store at -20°C.
• Materials Required but Not Supplied: Spectrophotometer/ Microplate reader, desk centrifuge, water-bath, adjustablepipette, micro quartz cuvette/
  96 well flat-bottom plate (UV plate) and distilled water.