NAD-Malate Dehydrogenase Activity Assay Kit I

• Cat.No.: KITZ-045
• Product Overview: Malate Dehydrogenase (MDH, EC 1.1.1.37)is widely exist in animal, plant, microbe and cells culture.
  MDH in mitochondria is one of the key enzymes of TCA cycle, which catalyzes the formation of
  oxaloacetic acid from malic acid. In contrast, MDH in serum catalyzes the formation of malic acid from
  oxaloacetic acid. Oxaloacetic acid is an important intermediate that connects several important metabolic
  pathways. MDH plays an important role in many physiological activities of cells, including mitochondrial
  energy metabolism, malic acid-aspartic acid shuttle system, reactive oxygen species metabolism and
  disease resistance. According to the different coenzyme specificity, MDH is divided into NAD- dependent
  MDH and NADP- dependent MDH. Bacteria usually only contain NAD-MDH. NAD-MDH is distributed
  in the cytoplasm and mitochondria in eukaryotic cells.
  NAD-MDH catalyzes NADH to reduce oxaloacetic acid into malic acid, which resulting in a decrease in
  absorption at 340nm.

Specification

• Size: 50T/48S
• Storage: Store at -20 °C
• Shipping: Ice pack
• Kit Components: Extract solution: 50 mL×1, store at 4°C.
  Reagent I: 40 mL×1, store at 4°C.
  Reagent II: Powder×2, store at -20°C. Add 360 μL of distilled water when the solution will be used. The
  rest of reagentstore at -20°C.
  Reagent III: Powder×2, store at -20°C. Add 327 μL of distilled water when the solution will be used. The
  rest of reagentstore at -20°C.
• Materials Required but Not Supplied: Ultraviolet spectrophotometer,desk centrifuge, water-bath, adjustablepipette, 1mL quartzcuvette, distilled
  water.