Mouse Monoclonal Antibody to Human Neuropilin And Tolloid-Like 1

• Cat.No.: CAB11586MH
• Product Overview: Mouse Monoclonal Antibody to Human Neuropilin And Tolloid-Like 1

Specification

• Species: Human
• Source: Mouse
• Tag: Non
• Antigen Description: Neuropilin and tolloid-like protein 1, also known as brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, NETO1 and BTCL1, is a single-pass type I membrane protein which contains two CUB domains and contains one LDL-receptor class A domain. Isoform 1 and isoform 2 of NETO1 / BTCL1 are retina-specific. Isoform 3 is found in retina as well as at lower levels in adult and fetal brain. NETO1 / BTCL1 interacts with PLZ domains of DLG2, DLG3 and DLG4 via its C-terminal TRV domain. It interacts with GRIN2A and GRIN2B via its CUB domains. NETO1 / BTCL1 is involved in the development and/or maintenance of neuronal circuitry. It is an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) critical for maintaining the abundance of GRIN2A-containing NMDARs in the postsynaptic density. NETO1 / BTCL1 regulates long-term NMDA receptor-dependent synaptic plasticity and cognition in the context of spatial learning and memory.
• Specificity: Human NETO1. No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA.
• Immunogen: Recombinant Human NETO1 Protein
• Isotype: Mouse IgG1
• Clone: 10B10F5
• Applications: Western blot; ELISA
• Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NETO1 in WB. Using a DAB detection system, the detection limit for Human NETO1 is approximately 8 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5-1 μg with the appropriate secondary reagents to detect Human NETO1. The detection limit for Human NETO1 is approximately 0.16 ng/well.
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human NETO1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: NETO1 neuropilin (NRP) and tolloid (TLL)-like 1 [ Homo sapiens ]
• Official Symbol: NETO1
• Synonyms: NETO1; neuropilin (NRP) and tolloid (TLL)-like 1; neuropilin and tolloid-like protein 1; BCTL1; BTCL1; brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1; FLJ41325
• Gene ID: 81832
• mRNA Refseq: NM_001201465
• Protein Refseq: NP_001188394
• MIM: 607973
• UniProt ID: Q8TDF5
• Chromosome Location: 18q22.2
• Function: receptor activity