Mouse Anti-Human Leu-5 Hybridoma
Cat.No. : | CSC-H0430 |
- Specification
- Gene Information
- Related Products
- Download
Species : | Human |
Tag : | Non |
Cell Line Description : | The antibody is cytotoxic for Leu-5 positive cells. The cells require NaP, oxaloacetic acid and bovine insulin in the growth medium. |
Introduction : | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-boxtype 1 and a B-boxtype 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies near the nucleus, but its function is unknown. The gene is located on chromosome 13 within the minimal deletion region for B-cell chronic lymphocytic leukemia. Alternative splicing of this gene results in three transcript variants which differ only in the 5 UTR. |
Immunogen : | Human lymphocytes (HLA phenotype A2, A28, B27, Bw4, Cw2) |
Immunological Donor : | BALB/c mouse spleen |
Fusion Species : | Mouse X Mouse Hybridoma |
Myeloma : | Sp2/0 |
Growth properties : | Suspension |
Morphology : | Lymphoblast |
Propagation : | Complete growth medium: RPMI 1640 + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 0. 05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37. 0 °C |
Subculturing : | Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. |
Mycoplasma : | Mycoplasma Status: Negative (MycoAlert Kit) |
Reactivity : | Anti-Leu-5 |
Isotype : | IgM |
Storage : | Liquid nitrogen |
Safety Considerations : | The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
Ship : | Dry ice |
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Ask a Question for All Leu-5 Products
Required fields are marked with *
My Review for All Leu-5 Products
Required fields are marked with *
0
Inquiry Basket