Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric)

• Cat.No.: Kit-2361
• Product Overview: Monoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity, and tissue localization. MAO-A can oxidize primary amines such as serotonin and norepinephrine. MAO-A is a mitochondrial-bound enzyme that is ubiquitously expressed throughout the brain and other tissues. It has been implicated in panic, anxiety, and depression. Several MAO-A specific inhibitors such as clorgyline, brofaromine, toloxatone, tetrindole, etc. have been used as antidepressants, but their usage has been limited due to side effects. MAO-A Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate.

Specification

• Applications: Screening/studying/characterizing MAO-A inhibitors
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: MAO-A Assay Buffer; OxiRed; Probe (in DMSO); MAO-A Enzyme (Lyophilized); MAO-A Substrate (Lyophilized); Developer (Lyophilized); Inhibitor Control (Clorgyline) (Lyophilized)
• Detection method: Fluorescence (Ex/Em = 535/587 nm)
• Features & Benefits: Rapid & sensitive;
  High-throughput assay to screen/study/characterize potential inhibitors of monoamine oxidase A;
  Includes Inhibitor Control [Clorgyline]
• Tag: Non