Mono-Avidin Beads

• Cat.No.: mono-avidin-13A
• Product Overview: Our Mono-Avidin Beads enable mild affinity purification of biotinylated proteins, peptides and other ligands. The mono-avidin molecules immobilized on the beads have a much lower biotin-binding affinity than native avidin thus enabling dissociation of biotinylated molecules using free biotin molecules or low pH buffer. In addition, the Mono-Avidin Beads can be regenerated at least 10 times with no significant loss in binding capacity.

Specification

• Tag: Non
• Cat.no: mono-avidin-13A
• Matrix: Highly cross-linked spherical agarose, 4%
• Functional group: Mono-Avidin
• Average particle size: 40-140 μm
• Coupling chemistry: Epoxy
• Dynamic binding capacity: 2-2.6 mg Avidin per ml of beads
• Recommended flow rate: 1-2 ml/min/cm2
• Elution buffer: 2 mM D-biotin in Phosphate buffer saline