MMP-3 Activity Assay Kit

• Cat.No.: Kit-0588
• Product Overview: The assay sensitivity is< 50 µU. (100 assays) Simple procedure; takes ~ 60 minutes;Fast and convenient;Sensitive assays for measuring MMP-3 in various biological samples.

Specification

• Size: 100 assays
• Description: The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development. In MMP-3 Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em = 325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is< 50 µU.
• Applications: The assay sensitivity is< 50 µU.
• Target Species: Mammalian
• Usage: This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
• Kit Components: MMP-3 Assay BufferMMP-3 SubstrateMca Standard (1mM)MMP-3 Positive Control
• Detection method: Fluorometer
• Compatible Sample Types: Cell Culture SupernatantsPlasmaSerumUrine
• Tag: Non