Mitochondrial Permeability Transition Pore Assay Kit

• Cat.No.: Kit-2286
• Product Overview: Mitochondria are the power centrals of the cell and play an essential role in energy production. Damage to mitochondria activates signaling pathways that induce apoptosis. The mitochondrial permeability transition pore (MPT pore or MPTP) is a nonspecific channel formed by components of the inner and outer mitochondrial membranes, and appears to be involved in the release of mitochondrial components during cell death. In healthy cells, MPTP’s flicker between open and closed states but during cell death MPTP’s dramatically alter the permeability of the mitochondria. Cytochrome c release and loss of mitochondrial membrane potential are subsequent to continuous pore activation. Mitochondrial Permeability Transition Pore Assay Kit provides a direct method of measuring cell death by measuring MPTP opening rather than relying on mitochondrial membrane potential alone.

Specification

• Applications: Measure mitochondrial permeability transition pore activity in cells
  Assessment of physiological mediators and antibodies that affect cell viability/cytotoxicity
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: MPTP Staining Dye; CoCl2; Ionomycin; MPTP Wash Buffer
• Target Species: Mammalian
• Detection method: Flow cytometry (Ex 488 nm)
• Features & Benefits: Simple procedure;
  Fast, convenient and sensitive;
  The assay provides a direct method of measuring mitochondrial permeability transition pore activation as an early marker of apoptosis
• Tag: Non