Methanol Assay Kit (fluorescence)

• Cat.No.: KITZ-040
• Product Overview: Methanol is found at very low levels in alcoholic beverages. Small amounts of methanol are present in normal, healthy human individuals. However, higher levels of methanol are toxic. Methanol poisoning typically occurs following ingestion of a methanol-containing product. The basis of the toxic effects of methanol is attributed to format, to which methanol is eventually converted. Elevated format levels can lead to confusion, blindness, and death. Due to its high toxicity, methanol is used as an additive in many industrial alcohol products, to prevent human consumption.
  Methanol Assay Kit provides simple and sensitive procedure for measuring methanol in various sample types. Methanol concentration is determined via a coupled enzymatic reaction. The fluorescence intensity, measured at λex=535nm/λem= 590nm, is proportional to the amount of methanol present in the sample. The linear ranges of this kit are 0.5–10nmol.

Specification

• Size: 200 fluorometric tests
• Storage: Store at -20 °C
• Shipping: Dry ice
• Kit Components: Assay Buffer 50 mL
  Methanol Standard 1 mL
  Enzyme I 100 μL
  Enzyme II 1 vial
  Developer 300 μL
  Probe 800 μL
• Materials Required but Not Supplied: 96-well black flat-bottom plates
  Fluorescence (λex = 535 nm/λem = 590 nm) plate reader
  Fume Hood