Mad2-p31(comet) complex

• Cat.No.: CBCRY03

Specification

• Cat. No.: CBCRY03
• Background: The crystal structure of the Mad2-p31(comet) complex is reported. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."
• Protein Classification: cell cycle
• Structure Weight: 105471.24 Da
• Polymer: 1
• Molecule: Mitotic spindle assembly checkpoint protein MAD2A
• Chain Length: 210 amino acids
• PDB ID: 2QYF
• Source: E.coli
• Method: X-Ray Diffraction
• Resolution: 2.30 Å
• Modified residues: selenomethionine (pareint: MET)
• Reference: Yang, M., Li, B., Tomchick, D.R., Machius, M., Rizo, J., Yu, H., Luo, X. p31comet blocks Mad2 activation through structural mimicry.Cell(Cambridge,Mass.)2007; 131: 744-755
• Tag: Non

Gene Information

• Gene Name: MAD2L1
• Synonyms: HSMAD2; MAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1
• UniProt ID: Q13257
• Chromosome Location: 4q27
• Function: protein binding
• Gene ID: 9587