MabAffinity rProtein G Agarose High Flow

• Cat.No.: Potein G-001A
• Product Overview: Mab Affinity Protein G Agarose High Flow Resin is a high capacity, chemically stable affinity medium designed specially for the purification of monoclonal and polyclonal antibodies and isolation of immune complex at laboratory and process scales.

Specification

• Tag: Non
• Cat.no: Potein G-001A
• Form: Slurry in 20% Ethanol
• Size: 5 mL, 25 mL, 100 mL
• Matrix: Highly cross-linked Agarose
• Molecular Characterization: Recombinant Protein G has a mass of 21.6kDa,while its apparent size is 31 to 34 kDa by SDS-PAGE
• Average particle size: 90 μm
• Ligand: Protein G
• Coupling chemistry: Epoxy
• Dynamic binding capacity: 18 mg IgG /ml media
• Recommended flow rate: 100-300cm/h
• Viscosity: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened and keep container tightly closed in a dry and well-entilated place.Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
• Recommended column height: 5‐20cm
• Chemical stability: Stable in all aqueous buffers commonly used in protein G chromatography
• pH working range: 2-9
• pH CIP range: 2-10
• CIP stability: 8M Urea, 6M Gua‐HCl, 3 M sodiumisothiocyanate, 0.1M acetic acid
• Temperature Stability: 2‐40 °C
• Elution buffer: 100 mM glycin, 10 mM NaCl, pH 3.0
• Shelf life: 5 years
• Warning: 1. Determined at 10% breakthrough at 4min residence time2. Delivered at room temperature, and recommended long‐term storage at 2‐8 °C