Lysine Agarose

• Cat.No.: Lysine-001A
• Product Overview: Lysine agarose 4B media with immobilized amino acid lysine is mainly used for isolation of plasminogen and plasminogen activator. The ε-amino and α-carboxyl groups on lysine is free after immobilization and interact with a range of biomolecules.

Specification

• Tag: Non
• Cat.no: Lysine-001A
• Characteristics: Lysine agarose 4B is L-lysine immobilized to agarose 4B by the cyanogen bromide method. L-lysine is coupled via its α-amino group, leaving both the ε-amino and α-carboxyl groups free to interact with sample substances during chromatography. It is designed for purification of molecules with biospecific or charge dependent affinity for L-lysine. Lysine agarose 4B is a group specific adsorbent and has been used for isolation of plasminogen and plasminogen activator, separation of ribosomal RNA (rRNA) and purification of doublestranded DNA. The precise mechanism by which separation occurs is not fully understood but both electrostatic and stereospecific effects may contribute to the separation, depending upon the application.
• Size: 15 g, 250 g
• Matrix: Agarose, 4%
• Functional group: Lysine
• Average particle size: 90 μm
• Ligand: L-lysine
• Ligand concentration: 4–7 μmol lysine/ml drained medium
• Dynamic binding capacity: 0.6 mg human plasminogen/ml drained medium; 0.6–0.7 mg rRNA/ml drained medium
• Chemical stability: Stable to all commonly used aqueous buffers
• Physical stability: Negligible volume variation due to changes in pH or ionic strength
• pH working range: 3-12
• pH CIP range: 2-13