Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

• Cat.No.: Kit-0542
• Product Overview: Simple procedure
  Fast and convenient
  Sensitive assay for measuring lipid peroxidation (LPA) in a wide range of samples

Specification

• Description: Quantification of lipid peroxidation is essential to assess oxidative stress in pathophysiological processes. Lipid peroxidation forms Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage. Lipid Peroxidation Assay Kit provides a convenient tool for sensitive detection of the MDA in a variety of samples. The MDA in the sample is reacted with Thiobarbituric Acid (TBA) to generate the MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (λ = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
• Applications: The kit detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
• Usage: For Research Use Only! Not For Use in Humans.
• Storage: -20°C
• Size: 100 assays
• Kit Components: • MDA Lysis Buffer
  • Phosphotungstic Acid Solution
  • BHT (100X)
  • TBA
  • MDA Standard (4.17M)
• Target Species: All
• Detection method: Absorbance (532 nm) or Fluorescence (Ex/Em 532/553 nm)
• Compatible Sample Types: Cell and Tissue culture supernatants, plasma and other biological fluids (optimized by end user).
• Tag: Non