L-Lactate Assay Kit

• Cat.No.: Kit-2464
• Product Overview: Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Specification

• Storage: -20ºC
• Shipping: On Ice
• Size: 100 tests
• Detection method: OD565nm
• Compatible Sample Types: Serum, plasma, cell culture media etc
• Features & Benefits: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay.
  Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
  High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
• Assay time: 20 min
• Sensitivity: 0.05 mM
• Tag: Non