Kappa Select Kit

• Cat.No.: Kappa-001A
• Product Overview: Antibody fragments, especially Fabs, are getting increased attention as potential biopharmaceuticals because they have some advantages over monoclonal antibodies (MAbs), KappaSelect is an affinity medium designed for the purification of human Fab (kappa) fragments.

Specification

• Tag: Non
• Cat.no: Kappa-001A
• Form: suspension in 20% ethanol
• Matrix: Highly cross-linked agarose
• Average particle size: 75 μm
• Ligand: Recombinant protein (Mr 13 000), produced in S. cerevisiae, with affinity for the constant domain of the immunoglobulin kappa light chain
• Ligand concentration: Approx. 5 mg/ml of medium
• Dynamic binding capacity: Approximately 15 mg Fab/mL of medium
• Recommended flow rate: At least 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20°C using buffers with the same viscosity as water at< 3="">
• Viscosity: We recommend storing the media in 20% ethanol at 2°C to 8°C. KappaSelect is supplied as suspension in 20% ethanol.
• Chemical stability: Stable to all commonly used aqueous buffers: 6 M urea, 6 M guanidine hydrochloride, 70 % ethanol, 30 % isopropanol
• pH working range: 3-10
• pH CIP range: 2-12
• Elution buffer: 0.1 M glycine buffer, pH 2.5–3.0
• Warning: Prolonged exposure (i.e., several days) to pH< 2="" should="" be="" avoided="" due="" to="" slow="" decomposition="" of="" the="" agarose="" matrix="" at="" low="" ph.="" for="" kappaselect="" avoid="" ph="">12