JMJD2C (KDM4C) Homogeneous Assay Kit

• Cat.No.: Kit-1773
• Product Overview: JMJD2C is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3.

Specification

• Size: 384 reactions
• Description: The JMJD2C Homogeneous Assay Kit is designed to measure activity of the JMJD2C for screening and profiling applications, using AlphaLISA technology. The key to the JMJD2C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Storage: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Kit Components: JMJD2C (GASC1, KDM4C): 60 µg; -80°C Primary antibody 5: 2 x 12.5 μl; -80°C Biotinylated histone H3 peptide substrate (JMJD2): 500 rxns; -80°C 4x HDM Assay Buffer 3: 2 ml; -20°C 4x HDM Incomplete assay buffer: 1 ml; -20°C 4x Detection buffer: 2 ml; -20°C
• Tag: Non