Isocitrate Fluorometric Assay Kit

• Cat.No.: Kit-0484
• Product Overview: Isocitrate Assay Kit (Fluorometric) is used for quantitative determination of Isocitrate (Isocitric Acid) using fluorometric methods.

Specification

• Description: ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.This isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λ ex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.
• Applications: Isocitrate determination in food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
• Usage: For research use only (RUO)
• Storage: Store all components at -20°C upon receiving.
• Kit Components: Assay Buffer 10 mLNADP 500 µLStandard 1 mLEnzyme A 120 µLEnzyme B 120 µLProbe 750 µL
• Detection method: Fluorometric
• Compatible Sample Types: Beverage, Food, Cell lysate, Serum, Tissue homogenate
• Features & Benefits: Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
• Tag: Non