IMAC Agarose Fast Flow

• Cat.No.: IMAC FF-001A
• Product Overview: IMAC agarose Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.

Specification

• Tag: Non
• Cat.no: IMAC FF-001A
• Characteristics: IMAC agarose Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC agarose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.IMAC agarose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
• Size: 25 mL, 100 mL
• Matrix: Highly cross-linked 6% spherical agarose
• Ion exchange capacity: Approx. 15 μmol Ni2+/ml medium
• Average particle size: 90μm
• Dynamic binding capacity: Approx. 40 mg (histidine)6-tagged protein/ml medium (Ni2+ charged).
• Recommended flow rate: <150>
• Recommended column height: 5‐20cm
• Chemical stability: Stable in: 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C. 1 M NaOH, 70% acetic acid. Tested for 12 h.2% SDS. Tested for 1 h. 30% 2-propanol. Tested for 30 min.
• pH working range: 3-12
• pH CIP range: 2-14
• Temperature Stability: 4-30°C