Human Spleen Cytoplasmic Lysate

• Cat.No.: Spleen-465H

Specification

• Species: Human
• Source: Human Spleen
• Tag: Non
• Concentration: 2 mg/mL or better
• Tissue Type: Spleen
• Shipping: Dry ice
• Preparation method: Human spleen tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human spleen tissue was frozen in liquid nitrogen immediately after excision and then stored at -70?C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated spleen tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated spleen tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.
• Diagnosis: Normal
• Storage Buffer: HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
• Storage Instruction: Store at 2-8?C for continuous use. For extended storage, freeze working aliquots at -70?C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
• Applications: Spleen cytoplasmic protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.