HDAC3 Fluorometric Assay Kit

• Cat.No.: KITZ-058
• Product Overview: The HDAC3 Fluorometric Assay Kit is a complete assay system designed to measure histone deacetylase 3 (HDAC3) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC3 activity measurements. In addition, the kit includes purified HDAC3 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

Specification

• Size: 96 reactions
• Storage: -80°C
• Shipping: Dry ice
• Kit Components: HDAC3 human recombinant enzyme: 2 μg, -80°C
  Fluorogenic HDAC substrate 3 (5mM): 25 μl, -80°C
  2x HDAC Developer (contains Trichostatin A) (2μM): 6 ml, -80°C
  Trichostatin A (200μM) in DMSO: 100 μl, -20°C
  HDAC Assay Buffer: 10 ml, -20°C
  Black, low binding NUNC black microtiter plate: 1 plate, Room Temp
  Avoid freeze/thaw cycles
• Materials Required but Not Supplied: 0.1% solution of bovine serum albumin (BSA) in water
  Fluorimeter capable of excitation at 350-380 nm and detection at 440-460 nm. Adjustable micropipettor and sterile tips
  Rotating or rocker platform