Halo Magnetic CoIP Agarose M-270 kit

• Cat.No.: Halo-05A
• Product Overview: The Halo-Trap Magnetic Particles M-270 Kit contains Halo-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficientImmunoprecipitation of Halo-tagged proteins and their interacting factors.
  • Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.
  • Ready-to-use
  • No heavy & light antibody chains on the SDS-PAGE
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis

Specification

• Specificity/Target: Halo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
• Binding capacity: 1.25 μg of recombinant Halo-tag per 25 μL bead slurry
• Conjugate: Magnetic Particles M-270, size: 2.8 μm
  high throughput-compatible
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 2 nM
• Storage Buffer: PBS with 0.09% sodium azide
• Applications: IP, CoIP, ChIP, RIP