Halo Magnetic CoIP Agarose beads

• Cat.No.: Halo-02A
• Product Overview: Immunoprecipitation of Halo-tagged proteins and their interacting factors with anti-Halo Nanobody conjugated to magnetic agarose beads.
  • Halo Trap recognizes Halo fusion proteins that are already bound to chloralkane-based ligands of the Halo-tag, e.g. dyes, biotin, etc.
  • Halo-Trap can be used for affinity purification
  • Bound Halo-fusion protein can be eluted without protease
  • Structure and function are characterized

Specification

• Specificity/Target: Halo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
• Binding capacity: 12.5 μg of recombinant Halo-tag per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 μm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 2 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP