Halo CoIP Agarose beads, kit

• Cat.No.: Halo-03A
• Product Overview: The Halo-Trap Agarose Kit contains Halo-Trap Agarose, lysis, wash, and elution buffers for efficientImmunoprecipitation of Halo-tagged proteins and their interacting factors.
  • Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.
  • Fast, reliable & efficient one-stepImmunoprecipitation
  • No heavy & light antibody chains
  • Bound Halo-fusion protein can be eluted without protease
  • Suitable for downstream mass spec analysis

Specification

• Specificity/Target: Halo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
• Binding capacity: 7.5-10 μg of recombinant Halo-tag per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 1 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 2 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP