Glyoxalase II Activity Kit (Colorimetric)

• Cat.No.: Kit-2337
• Product Overview: Glyoxalase II (GloII, hydroxyacylglutathione hydrolase, EC 3.1.2.6) is one of the two enzymes in the Glyoxalase system that is ubiquitously expressed in mammalian, plants and bacteria. The Glyoxalase system is a biological pathway that detoxifies cells by metabolizing α-ketoaldehydes such as methylglyoxal, a cytotoxic byproduct of lipids and glucose metabolism. GloII fulfills the terminal step in the glyoxalase system: It catalyzes the hydrolysis of S-D-lactoyl-glutathione (SLG) to reduced glutathione and D-lactic acid. The alteration of GloII activity in cell growth cycle, cell differentiation, phagocyte activation and diabetes mellitus development of implies this enzyme plays a wide-ranging and important role in cell function and disease progression. Research areas of current interest in Glo-II include diabetes, and cancer therapy. Glyoxalase II Activity Kit utilizes the ability of an active Glo-II to cleave a substrate while producing D-lactate. The produced D-Lactate reacts with the chromophore generating a stable signal that can be easily quantified at 450 nm using a microplate reader. Our assay kit is simple, sensitive and can detect as low as 0.6 mU/ml of Glo-II activity in biological samples.

Specification

• Tag: Non
• Applications: Measurmente of Glyoxalase II activity in biological samples
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: GloII Assay Buffer; Enzyme Mix (Lyophilized); GloII Substrate (Lyophilized); GloII Probe (Lyophilized); GloII Positive Control; D-Lactate Standard (100 mM)
• Target Species: Mammalian
• Detection method: Colorimetric (OD 450 nm)
• Features & Benefits: Simple procedure; takes ~ 40 minutes;
  Fast and convenient;
  Kit contains all necessary reagents for accurate measurement ofGlyoxalase II