Glutathione Beads

• Cat.No.: Glutathione-30A
• Product Overview: Glutathione Beads are used for the purification of glutathione Stransferase (GST) tagged proteins, produced using various commercial expression vectors. GST-fused proteins can be purified from bacterial lysates by one-step affinity purification. Our Glutathione Beads is state of the art affinity-resin product that consists of glutathione attached through sulfur to an epoxy-activated 4% cross-linked beaded agarose. The use of the Glutathione Beads enables the purification of various GSTfused proteins, while maintaining mild, non-denaturing conditions throughout the purification process.

Specification

• Cat.no: Glutathione-30A
• Matrix: Highly cross-linked spherical agarose, 4%
• Average particle size: 45‐165 μm
• Dynamic binding capacity: 4-8 mg/ml recombinant GST
• Recommended flow rate: 1-2 ml/min/ cm2
• pH working range: 4-10
• Elution buffer: 10 mM reduced Glutathione in 50mM Tris-HCl, pH 8
• Tag: Non