Glucose Uptake Fluorometric Assay Kit

• Cat.No.: Kit-2250
• Product Overview: Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.

Specification

• Applications: This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: Extraction Buffer; Neutralization Buffer; 2-Deoxyglucose (2-DG, 10 mM); 2-DG Uptake Assay Buffer; Enzyme Mix (Lyophilized); (in DMSO); 2-DG6P Standard (Lyophilized)
• Target Species: Mammalian
• Detection method: Fluorescence(Ex/Em = 535/587 nm)
• Features & Benefits: Simple procedure;
  Fast and convenient;
  The assay is easy, non-radioactive, and highly sensitive
• Tag: Non