Glucose Dehydrogenase Assay Kit

• Cat.No.: Kit-2523
• Product Overview: GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway. The non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Specification

• Storage: RT
• Shipping: RT
• Size: 100 tests
• Detection method: OD565nm
• Compatible Sample Types: Biological samples (e.g. plasma, serum, tissue and culture media).
• Features & Benefits: Fast and sensitive. Linear detection range (20 μL sample): 0.5 to 200 U/L for 15 min reaction.
  Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
• Assay time: 15 min
• Sensitivity: 0.5 U/L
• Tag: Non