Glucose-6-Phosphate Dehydrogenase Kit

• Cat.No.: Kit-2450
• Product Overview: GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. The non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Specification

• Tag: Non
• Storage: -20ºC
• Shipping: RT
• Size: 100 tests
• Detection method: OD460nm
• Compatible Sample Types: Plasma, serum, tissue and culture media, etc
• Features & Benefits: Fast and sensitive. Linear detection range (20 μL sample): 0.2 to 100 U/L for 15 min reaction.
  Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
• Assay time: 20 min
• Sensitivity: 0.2 U/L