Glucoamylase Assay Kit

• Cat.No.: KITZ-051
• Product Overview: Glucoamylase (EC3.2.1.3) also known as Saccharifying enzyme, which is an extracellular
  enzyme that is secreted by a series of microorganisms and has exonuclease activity. The
  
  main role of glucoamylase is to hydrolyze α-1,4 glycosidic bonds in sequence from non-
  reducing ends on the carbon chains such as starch, dextrin, glycogen, etc., and cut off each
  
  glucose unit. For amylopectin, when it encounters a branch point, it can also hydrolyze
  α-1,6 glycosidic bonds, thereby hydrolyzing all amylopectin to glucose. It is one of the
  important industrial enzyme preparations, and is widely used in alcohol, liquor, antibiotics,
  amino acids, organic acids, glycerol, starch sugar and other industries.
  Soluble starch could be hydrolyzed into glucose by glucoamylase. 3, 5-Dinitrosalicylic acid
  is reduced to brown-red amino compound by co-heating with glucose under alkaline
  condition. The brown-red amino compound has a maximum absorption peak at 540 nm and
  the absorbance ratio is in direct proportion to the contents of glucose. The glucoamylase
  activity is quantified by measuring the color development at 540 nm.

Specification

• Size: 50T
• Storage: 2-8°C
• Shipping: Ice pack
• Kit Components: Extract solution: Liquid 110 mL×1. Store at 2-8°C.
  Reagent I: Powder×2. Store at 2-8°C. Add 20 mL of Extract solution to one Reagent I before use. Mix thoroughly, then place in a boiling water bath for 10 minutes to make it fully dissolved. It could be stored at 2-8°C for four weeks.
  Reagent II: Liquid 35 mL×1. Store at 2-8°C and protect from light.
  Standard: Powder×1. Store at 4°C. Dissolve the standard with 1 mL of Extract solution to generate a 10 mg/mL glucose solution standard. It could be stored at 2-8°C for two weeks.
• Materials Required but Not Supplied: Spectrophotometer, water bath, balance, centrifuge, adjustable transferpettor, mortar/homogenizer/cell ultrasonic crusher, 1 mL glass cuvette, ice and distilled water.