GFP CoIP Agarose

• Cat.No.: GFP-01A
• Product Overview: Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.
  • Fast, reliable & efficient one-stepImmunoprecipitation
  • Ready-to-use
  • No heavy & light antibody chains
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis
  • Works in samples from: mammals, plants, bacteria, yeast, insects etc.

Specification

• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
• Binding capacity: 25-30 μg of recombinant GFP per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 1 pM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP
• Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!