EHMT1/GLP Chemiluminescent Assay Kit

• Cat.No.: Kit-1721
• Product Overview: EHMT1 (GLP) is a histone methyltransferase that exhibits methylation activity toward H3-K9.

Specification

• Size: 96 reactions
• Description: The EHMT1 (GLP) Direct Activity Chemiluminescent Assay Kit is designed to measure EHMT1, also known as G9a-like protein or GLP activity for screening and profiling applications. The key to the EHMT1 (GLP) Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K9 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primaryantibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Storage: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Kit Components: EHMT1 (GLP): 4 µg; -80°CPrimary antibody 5: 12.5 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°C20 µM S-adenosylmethionine : 250 µl; -80°C4x HMT assay buffer 1: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate (2 components): 6 ml each; +4°C 96-well plate precoated with histone substrate: 1 plate; +4°C
• Tag: Non