DYKDDDDK Fab CoIP Agarose

• Cat.No.: DYKDDDDK Fab-01A
• Product Overview: The DYKDDDDK Fab-Trap Agarose is a premium affinity resin for efficientImmunoprecipitation (IP) of Flag-tagged proteins.The DYKDDDDK Fab-Trap Agarose consists of a Fab-fragment, which is covalently bound to agarose beads.
  • Lowest background in class
  • Less contaminating antibody chains
  • Recombinantly expressed Fab-fragment derived from M2 clone
  • Compatible with harsh buffer conditions
  • Ready-to-use

Specification

• Specificity/Target: The DYKDDDDK Fab-Trap Agarose specifically binds to 1x and 3xDYKDDDDK-tag sequence (1x and 3xFlag-tag). It binds to same epitope as Sigma''s Anti-FLAG M2 Antibody and is compatible with N-, C-terminal or internal tagging.
• Binding capacity: 12.5 - 15 μg of recombinant 3xDYKDDDDK-tagged protein (~30 kDa) per 25μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: 3xDYKDDDDK-peptide
  SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 0.5 % Nonidet P40 Substitute, 5 % Tween™ 20, 2 M NaCl, 10 mM TCEP, 10 mM DTT, 10 mM ß-mercaptoethanol, 2 M Urea
• Type: Nanobody
• Storage Buffer: 1x PBS, Preservative: 0.09 % sodium azide
• Applications: IP, CoIP, ChIP, RIP, Protein purification
• Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!