Diamine Oxidase (DAO) Activity Assay Kit

• Cat.No.: KITZ-034
• Product Overview: DAO (EC1.4.3.6) is widely found in animals (intestinal mucosa, lung, liver, kidney, etc.), plants, and
  microorganisms. Catalytic oxidation of polyamines to aldehydes, whose activity is closely related to
  nucleic acid and protein synthesis, can reflect the integrity and damage degree of the intestinal mechanical
  barrier.
  DAO catalyzed cadaverine to produce aldehydes and hydrogen peroxide, and Fe2+ in potassium
  ferricyanide oxidized by H2O2 to produce Fe3+. Fe3+ reacted with 4-amino-antipyrine and phenol to
  produce red quinones with characteristic absorption peaks at 505nm. The activity of DAO was calculated
  by measuring the absorbance value at 505nm.

Specification

• Size: 100T/48S
• Storage: 2-8°C
• Shipping: On Ice
• Kit Components: Extraction reagent: Liquid 60 mL×1. Store at 2-8°C.
  Reagent I: Liquid 2 mL×1. Store at 2-8°C.
  Reagent Ⅱ: Liquid 15 mL×1. Store at 2-8°C.
  Reagent Ⅲ: Liquid 5 mL×1. Store at 2-8°C.
  Standard goods: Liquid 102 μL×1. Store at 2-8°C. 1mol/L (1000μmol/mL) hydrogen peroxide
  solution was obtained by adding 898μL distilled water before use and stored at 2-8°C for 4 weeks.
• Materials Required but Not Supplied: Spectrophotometer/Microplate reader, mortar/homogenizer/cell ultrasonic crusher, centrifuge,
  constant temperature foster box/water-bath, centrifuge, micro quartz cuvette/96 well flat-bottom plate, ice
  and distilled water.