Recombinant Yeast TRR1

• Cat.No.: TRR1-1907Y
• Product Overview: Recombinant Yeast Thioreduxin Reductase produced inE.coliis a single, non-glycosylated, polypeptide chain, 36kDa.

Specification

• Species: Yeast
• Source: E.coli
• Tag: Non
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Ala-Met-His-Val.
• Form: Purified, in PBS, pH 7.4.
• Purity: >95%, as determined by: (a) Analysis by RP-HPLC and anion-exchange FPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Dimers & Aggregates: Less than 1% as determined by silver stained SDS-PAGE gel analysis.
• Endotoxin: Less than 0.1 ng/µg (IEU/µg) of ryNTR.
• Protein Content: Protein quantitation was carried out by two independent methods: 1) UV spectroscopy at 280 nm. 2) Analysis by RP-HPLC, using a standard solution of CTLA-4 as a Reference Standard.
• Application: 1) Molecular standard (Western, ELISA) in studying secreted BMP-4. 2) Preparing antibodies for BMP-4 monomer. 3) Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.
• Biological Activity: RyNTR is fully biologically active when compared to standardt Definition: One unit equals the change in absorbance at 412 nm per ute at 25°C using 0.2mM NADPH containing 5mM DTNB (pH 7.0) specific activity was found to be 5IU/mg.
• Storage: -20°C (aliquot), avoid freezing and thawing cycles.
• Pathways: Pyrimidine metabolism

Gene Information

• Gene Name: TRR1 Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress [ Saccharomyces cerevisiae ]
• Synonyms: Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; TRR1;Cytoplasmic thioredoxin reductase, key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress Trr1p
• Gene ID: 851955
• Protein Refseq: NP_010640
• Chromosome Location: IV
• Function: FAD binding; ferrous iron binding; oxidoreductase activity; thioredoxin-disulfide reductase activity