Active Recombinant Staphylococcal Spa

• Cat.No.: spa-1286S
• Product Overview: Recombinant Staphylococcal Protein A produced inE.Coliwas a non-glycosylated, Polypeptide chain having a molecular mass of 45 kDa. Recombinant Staphylococcal Protein A is purified by proprietary chromatographic techniques.

Specification

• Species: Staphylococcus
• Source: E.coli
• Tag: Non
• Description: Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E.coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin).
• Physical Appearance: Sterile Filtered clear colorless solution.
• Formulation: The protein solution contains no additives.
• Activity: Greater than 95.0% binding activity to human IgG.
• Purity: Greater than 98.0% as determined by RP-HPLC.
• Stability: SPA should be stored at -20°C.

Gene Information

• Gene Name: spa immunoglobulin G binding protein A precursor (protein A) [ Staphylococcus aureus subsp. aureus str. Newman ]
• Synonyms: spa; immunoglobulin G binding protein A precursor (protein A)
• Gene ID: 5332074
• Protein Refseq: YP_001331090