Active Recombinant Full Length E.coli FKBP-type Peptidyl-prolyl Cis-trans Isomerase/SlyD Protein

• Cat.No.: SlyD-329E
• Product Overview: Recombinant E.Coli slyD was expressed in E.coli and purified by conventional chromatography techniques (196 aa, 21 kDa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: Non
• Protein Length: 1-196 a.a.
• Description: SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5).
• Molecular Weight: 21 kDa (196 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
• Biological activity: Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
• Activity Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant SlyD protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: slyD FKBP-type peptidyl-prolyl cis-trans isomerase [ Escherichia coli CFT073 ]
• Synonyms: slyD; FKBP-type peptidyl-prolyl cis-trans isomerase; slyD FKBP-type peptidyl-prolyl cis-trans isomerase.
• Gene ID: 1036353
• Protein Refseq: NP_755987.1
• UniProt ID: P0A9L0