Active Native Bacillus stearothermophilus Acetate Kinase

• Cat.No.: AK-14B
• Product Overview: Native Acetate Kinase was purified from Bacillus stearothermophilus. EC 2.7.2.1

Specification

• Species: Bacillus stearothermophilus
• Source: Bacillus stearothermophilus
• Tag: Non
• Description: In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. The enzyme is important in the process of glycolysis, enzyme levels being increased in the presence of excess glucose. The growth of a bacterial mutant lacking acetate kinase has been shown to be inhibited by glucose, suggesting that the enzyme is involved in excretion of excess carbohydrate. A related enzyme, butyrate kinase, facilitates the formation of butyryl-CoA by phosphorylating butyrate in the presence of ATP to form butyryl phosphate.
• Form: Lyophilized
• Bio-activity: more than 1,100 U/mg protein
• Molecular Mass: Molecular weight : ca. 160,000 Subunit molecular weight : ca. 40,000
• Unit Definition: One unit of activity is defined as the amount of AK that forms 1 μmol of ADP per minute at 30°C.
• Applications: The enzyme is useful for determination of acetate or for ATP regeneration system.
• Stability: No detectable decrease in activity up to 65°C.
• Storage: stable at -20°C for at least one year.