Recombinant E.coli acetate kinase A and propionate kinase 2, His-tagged

• Cat.No.: ackA-621E
• Product Overview: ackA, 1-400aa, E.coli, His tag, E.coli

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Protein Length: 1-400 a.a.
• Description: ackA catalyzes the formation of acetyl phosphate from acetate and ATP. This protein can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. Recombinant E.coli ackA protein, fused to His-tag at N-terminus, was expressed in E.coli
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 45.7kDa (423aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSKLVL VLNCGSSSLK FAIIDAVNGE EYLSGLAECF HLPEARIKWK MDGNKQEAAL GAGAAHSEAL NFIVNTILAQ KPELSAQLTA IGHRIVHGGE KYTSSVVIDE SVIQGIKDAA SFAPLHNPAH LIGIEEALKS FPQLKDKNVA VFDTAFHQTM PEESYLYALP YNLYKEHGIR RYGAHGTSHF YVTQEAAKML NKPVEELNII TCHLGNGGSV SAIRNGKCVD TSMGLTPLEG LVMGTRSGDI DPAIIFHLHD TLGMSVDAIN KLLTKESGLL GLTEVTSDCR YVEDNYATKE DAKRAMDVYC HRLAKYIGAY TALMDGRLDA VVFTGGIGEN AAMVRELSLG KLGVLGFEVD HERNLAARFG KSGFINKEGT RPAVVIPTNE ELVIAQDASR LTA
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ackA?acetate kinase A and propionate kinase 2 [?Escherichia coli str. K-12 substr. MG1655?]
• Official Symbol: ackA
• Synonyms: ackA; ECK2290; JW2293; acetate kinase A and propionate kinase 2; NP_416799.1; EC 2.7.2.-; EC 2.7.2.1; acetate kinase
• Gene ID: 946775
• Protein Refseq: NP_416799
• Pathway: Carbon metabolism; Metabolic pathways; Microbial metabolism in diverse environments