Recombinant E.coli glycerol dehydrogenase, NAD, His-tagged

• Cat.No.: gldA-601E
• Product Overview: gldA, 1-367aa, E. coli, His tag, E.coli

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Protein Length: 1-367 a.a.
• Description: gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively. Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In Phosphate buffered saline (pH7.4), 10% glycerol
• Molecular Mass: 41.1 kDa (390aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: gldA?glycerol dehydrogenase, NAD [?Escherichia coli str. K-12 substr. MG1655?]
• Official Symbol: gldA
• Synonyms: gldA; ECK3937; JW5556; glycerol dehydrogenase, NAD; NP_418380.4; EC 1.1.1.6; glycerol dehydrogenase, (NAD)
• Gene ID: 948440
• Protein Refseq: NP_418380
• Pathway: Glycerolipid metabolism; Metabolic pathways; superpathway of methylglyoxal degradation