Active Recombinant Saccharomyces Cerevisiae GAL4, His-tagged

• Cat.No.: GAL4-8480S
• Product Overview: Recombinant Saccharomyces Cerevisiae GAL4, fused with His tag, was expressed in E. coli.

Specification

• Species: Yeast
• Source: E.coli
• Tag: His
• Description: Transcriptional activity is greatly stimulated by promoter-specific activator proteins . These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain . The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5-CGGN5(T/A)N5CCG-3 . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription . Linking of an acidic synthetic peptide, forming an ?-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure . This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
• AA Sequence: MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD NVNKDAVTDR LASVETDMPL TLRQHRISAT SSSEESSNKG QRQLTVSELN ELNELNALLN NN
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 300 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: GAL4 Gal4p [ Saccharomyces cerevisiae S288c ]
• Official Symbol: GAL4
• Synonyms: GAL4; GAL81; Gal4p
• Gene ID: 855828
• mRNA Refseq: NM_001184062
• Protein Refseq: NP_015076
• UniProt ID: P04386
• Chromosome Location: chromosome: XVI