Recombinant Human Acyl-CoA Thioesterase 7, His-tagged

• Cat.No.: ACOT7-1340H
• Product Overview: Recombinant human ACOT7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ACOT7 is a member of the acyl coenzyme family. This protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this protein may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol, 0.15 M NaCl.
• Molecular Weight: 42.6 kDa (390 aa), confirmed by MALDI-TOF
• Purity: > 95 % by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MARPGLIHSA PGLPDTCALL QPPAASAAAA PSMSGPDVET PSAIQICRIM RPDDANVAGN VHGGTILKMI EEAGAIISTR HCNSQNGERC VAALARVERT DFLSPMCIGE VAHVSAEITY TSKHSVEVQV NVMSENILTG AKKLTNKATL WYVPLSLKNV DKVLEVPPVV YSRQEQEEEG RKRYEAQKLE RMETKWRNGD IVQPVLNPEP NTVSYSQSSL IHLVGPSDCT LHGFVHGGVT MKLMDEVAGI VAARHCKTNI VTASVDAINF HDKIRKGCVI TISGRMTFTS NKSMEIEVLV DADPVVDSSQ KRYRAASAFF TYVSLSQEGR SLPVPQLVPE TEDEKKRFEE GKGRYLQMKA KRQGHAEPQP
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Biosynthesis of unsaturated fatty acids; Fatty acid elongation

Gene Information

• Gene Name: ACOT7 acyl-CoA thioesterase 7 [ Homo sapiens ]
• Official Symbol: ACOT7
• Synonyms: ACOT7; acyl-CoA thioesterase 7; ACT; ACH1; BACH; LACH; LACH1; hBACH; CTE-II; MGC1126; cytosolic acyl coenzyme A thioester hydrolase; CTE-IIa; OTTHUMP00000001410; OTTHUMP00000001413; OTTHUMP00000001414; OTTHUMP00000001415; OTTHUMP00000200492; OTTHUMP00000200493; acyl-CoA thioesterase 2; brain acyl CoA hydrolase; brain acyl-CoA hydrolase; acyl-CoA thioesterase, long chain; long chain acyl-CoA thioester hydrolase; NP_009205.3; EC 3.1.2.2
• Gene ID: 11332
• mRNA Refseq: NM_007274
• Protein Refseq: NP_009205
• MIM: 602587
• UniProt ID: O00154
• Chromosome Location: 1p36
• Function: acyl-CoA hydrolase activity; carboxylesterase activity; catalytic activity; fatty-acyl-CoA binding; hydrolase activity; palmitoyl-CoA hydrolase activity