Recombinant E. coli HchA, His-tagged

• Cat.No.: hchA-3513E
• Product Overview: Recombinant E. colihchA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-283aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: HchA, also known as,Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol0.1M NaCl,1mM DTT
• Molecular Weight: 33.3 kDa(303aa) confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1.0 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFSTGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIGLPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGRVHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: hchA Hsp31 molecular chaperone [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: Hsp31 molecular chaperone; ECK1963; JW1950; yedU; yzzC
• Gene ID: 946481
• Protein Refseq: NP_416476