Active Recombinant Human MAM Domain Containing Glycosylphosphatidylinositol Anchor 2, His-tagged

• Cat.No.: MDGA2-5687H
• Product Overview: Recombinant Human MDGA2protein was expressed in Murine myeloma cell line with a C-terminal 6-His tag. Gln21-Asp931.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 21-931 a.a.
• Description: MDGA2 is a 130 kDamember of the Ig superfamily of proteins. Human MDGA2 is synthesized as a 956amino acid (aa) precursor that contains a 25 aa signal sequence, a 906 aamature chain, and a 25 aa propeptide. The mature chain consists of sixIg-like domains, followed by a MAM domain (aa 746-921) and a GPI anchor. Inaddition, there are eight potential sites for N-linked glycosylation. Maturehuman MDGA2 shares 98% aa sequence identity with mature mouse and rat MDGA2.MDGA2 is structurally similar to other IgCAMS, such as the L1 family andaxonin 1, which have roles in cell adhesion, migration, and processoutgrowth.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Gln21 predicted
• Molecular Weight: 103.2kDa
• Activity: Measuredby its ability to enhance neurite outgrowth of E18 rat embryonic corticalneurons. Able to significantly enhance neurite outgrowth when immobilized at7.5-30 μg on a nitrocellulose-coated microplate.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 105-125kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 200μg/mL in sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: MDGA2 MAM domain containingglycosylphosphatidylinositol anchor 2 [ Homo sapiens ]
• Official Symbol: MDGA2
• Synonyms: MDGA2; MAM domaincontaining glycosylphosphatidylinositol anchor 2; MAMDC1; c14_5286; MAMdomain-containing glycosylphosphatidylinositol anchor protein 2; MAM domaincontaining 1; MAMDC1; OTTHUMP00000028200; OTTHUMP00000179128; MAMdomain-containing protein 1; UNQ8188/PRO23197
• Gene ID: 161357
• mRNA Refseq: NM_182830
• Protein Refseq: NP_878250
• MIM: 611128
• UniProt ID: Q7Z553
• Chromosome Location: 14q21.2