Recombinant Human Kinase Insert Domain Receptor (a type III receptor tyrosine kinase), Fc-tagged

• Cat.No.: KDR-5442H
• Product Overview: Recombinant human KDRwas fused with the Fc part of human IgG1. The recombinant mature KDRis a disulfide-linked homodimeric protein. The KDR monomers have a mass ofapproximately 160 kDa.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Fc
• Description: KDR has a lower affinity for VEGF than theFlt-1 receptor, but a higher signaling activity. Mitogenic activity inendothelial cells is mainly mediated by KDR leading to their proliferation.Differential splicing of the flt-1 gene leads to the formation of a secreted,soluble variant of VEGFR-1 (sVEGFR-1). No naturally occurring, secreted formsof KDR have so far been reported. The binding of VEGF165 to KDR is dependenton heparin.
• Form: Lyophilized
• Molecular Weight: 160 kDa
• Purity: > 90%, bySDS-PAGE and visualized by silver stain
• Endotoxin Level: < 0.1 ng per ugof KDR
• Biological Activity: The activity of KDRwas determined by its ability to inhibit the VEGF-dependent proliferation ofhuman umbilical vein endothelial cells. The ED50 for this effectis typically 10-30 ng/ml.
• Reconstitution: The lyophilized KDRis soluble in water and most aqueous buffers. The lyophilized KDR should bereconstituted in PBS or medium to a concentration not lower than 50 ug/ml.
• Storage: Lyophilizedsamples are stable for greater than six months at -20°C to -70°C.Reconstituted KDR should be stored in working aliquots at -20°C. Avoidrepeated freeze-thaw cycles.

Gene Information

• Gene Name: KDR kinase insert domain receptor (a typeIII receptor tyrosine kinase) [ Homo sapiens ]
• Official Symbol: KDR
• Synonyms: KDR; kinase insertdomain receptor (a type III receptor tyrosine kinase); FLK1;CD309; VEGFR; VEGFR2; vascular endothelial growth factor receptor 2; solubleVEGFR2; OTTHUMP00000158928; fetal liver kinase 1; fetal liver kinase-1;protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factorreceptor
• Gene ID: 3791
• mRNA Refseq: NM_002253
• Protein Refseq: NP_002244
• MIM: 191306
• UniProt ID: P35968
• Chromosome Location: 4q11-q12
• Pathway: Cytokine-cytokine receptorinteraction; Endocytosis; Focal adhesion; VEGF signaling pathway; Signalingby VEGF
• Function: ATP binding; growth factorbinding; nucleotide binding; receptor activity; transferase activity;vascular endothelial growth factor receptor activity