DEAE Cellulose Resin

• Cat.No.: diethylaminoethyl-004C
• Product Overview: DEAE-Cellulose is a cellulose ion-exchange adsorbent used in ion exchange chromatography.

Specification

• Tag: Non
• Description: CAS number is 9013-34-7.
  MDL number: MFCD00146622
• Form: Solid
• Usage: Provided as a dry resin.
  The following procedure should be carried out in a Buchner funnel:
  Suspend dry resin in 5 volumes diH2O and allow to swell and settle for 30-45 minutes. Measure volume of settled resin to determine Column Volume (CV) for washing steps.
  
  For activation/regeneration:
  1, Filter suspension and re-suspend resin in 2 CV of 0.1M NaOH/0.5M NaCl for 10 minutes. Pour slurry into Buchner funnel and allow buffer to slowly drain off (～1 V buffer in 5 minutes);
  2, Repeat using 0.5M NaCl (without 0.1M NaOH);
  3, Repeat using 0.1M HCl/0.5 M NaCl;
  4, Repeat using diH2O;
  5, Continue washing with diH2O until effluent pH is 5 or greater (~5-10 CV);
  6, Suspend the resin in 2 CV of 1M NaCl and adjust pH to 7-8 with NaOH and store at 4°C.
  
  To Use Resin:
  1, Filter resin and pass 5 CV of diH2O through resin;
  2, Resuspend in 2 CV of 10X buffer of your choice and filter;
  3, Remove resin from funnel and re-suspend with 5 CV of 1X buffer and filter. Wash with 1X buffer until pH is within 0.15 pH units of the 1X buffer;
  4, Pack resin into column via gravity flow;
  5, Apply sample, wash resin, and elute sample.
• Storage: Store at 4 centigrade.

Gene Information

• Synonyms: Diethylaminoethyl-cellulose