CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit
Cat.No. : | Kit-1712 |
Product Overview : | The activation of native T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in an inhibitory signal and prevents T-cell activation. CTLA4:B7-1 interaction is an important drug target for the regulation of the host"s response to cancer. |
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Tag : | Non |
Size : | 96 reactions |
Description : | The CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CTLA4 is coated on a 96-well plate. Next, B7-1 is incubated with CTLA4 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader. |
Applications : | This kit is useful for screening for inhibitors of B7-1 binding to CTLA4. |
Storage : | Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. |
Kit Components : | B7-1, Biotin-labeled: 5 µg; -80°C CTLA4: 10 µg; -80°C Streptavidin-HRP: 15 µl; +4°C 3x CTLA4 Assay Buffer: 50 ml; -20°C Blocking Buffer: 50 ml; +4°C HRP chemiluminescent substrate A (transparent bottle): 6 ml; +4°C HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C White 96-well microplate: 1; +4°C |
◆ Recombinant Proteins | ||
CTLA4-2232H | Recombinant Human CTLA4 protein, His-tagged | +Inquiry |
Ctla4-3307MAF647 | Recombinant Mouse Ctla4 Protein, His-tagged, Alexa Fluor 647 conjugated | +Inquiry |
CTLA4-2232HAF555 | Recombinant Human CTLA4 Protein, His-tagged, Alexa Fluor 555 conjugated | +Inquiry |
CTLA4-1954M | Active Recombinant Mouse CTLA4 protein, hFc-tagged | +Inquiry |
CTLA4-2075H | Recombinant Human CTLA4 Protein, GST-tagged | +Inquiry |
◆ Cell & Tissue Lysates | ||
CTLA4-2179MCL | Recombinant Mouse CTLA4 cell lysate | +Inquiry |
CTLA4-2526HCL | Recombinant Human CTLA4 cell lysate | +Inquiry |
CTLA4-1047CCL | Recombinant Cynomolgus CTLA4 cell lysate | +Inquiry |
Not For Human Consumption!
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